Deep learning-based cytoskeleton segmentation for accurate high-throughput measurement of cytoskeleton density. Ryota Horiuchi, Asuka Kamimura, Yuga Hanaki, Hikari Matsumoto, Minako Ueda, Takumi Higaki. Protoplasma 2025/5

The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis. Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu. The Plant Cell 2025/4/2

Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures. Manami Ichita, Haruna Yamamichi, Takumi Higaki. Plant Molecular Biology 202/2

Distinct localization patterns of actin microfilaments during early cell plate formation in plants through deep learning-based image restoration. Suzuka Kikuchi, Takumi Kotaka, Yuga Hanaki, Minako Ueda, Takumi Higaki. 2025/1/30

Morphological and Transcriptome Analysis of the Near-Threatened Orchid Habenaria radiata with Petals Shaped Like a Flying White Bird. Seiji Takeda, Yuki Nishikawa, Tsutomu Tachibana, Takumi Higaki, Tomoaki Sakamoto, Seisuke Kimura. Plants 2025/1/28

Machine Learning Accelerates Screening of Osteoclast Differentiation Inhibitors from Natural Products. Yuki Hitora, Mako Hokaguchi, Yusaku Sadahiro, Takumi Higaki, Sachiko Tsukamoto. Journal of natural products 2024/10/4

The PATROL1 function in roots contributes to the increase in shoot biomass. Michitaka Notaguchi, Manami Ichita, Takaya Kawasoe, Keina Monda, Ken-ichi Kurotani, Takumi Higaki, Koh Iba, Mimi Hashimoto-Sugimoto. Planta 260(5) 105-105 2024/9/26

At2-MMP is required for attenuation of cell proliferation during wound healing in incised Arabidopsis inflorescence stems. Afiifah Machfuudzoh, Weerasak Pitaksaringkarn, Ryo Koshiba, Takumi Higaki, Randeep Rakwal, Yusuke Ohba, Masashi Asahina, Shinobu Satoh, Hiroaki Iwai. Plant & cell physiology 2024/9/13

HD-ZIP IV genes are essential for embryo initial cell polarization in the radial axis initiation inArabidopsis. Sayuri Tanaka, Yuuki Matsushita, Yuga Hanaki, Takumi Higaki, Naoya Kamamoto, Katsuyoshi Matsushita, Tetsuya Higashiyama, Koichi Fujimoto, Minako Ueda. Current biology : CB 2024/4/29

Neopetromin, a Cyclic Tripeptide with a C-N Cross-Link, from the Marine Sponge Neopetrosia sp., That Causes Vacuole Fragmentation in Tobacco BY-2 Cells. Yuki Hitora, Ahmed H El-Desoky, Yusaku Sadahiro, Ai Sejiyama, Amiri Kinoshita, Yuji Ise, Esther D Angkouw, Remy E P Mangindaan, Takumi Higaki, Sachiko Tsukamoto. Journal of natural products 87(4) 1197-1202 2024/4/26

Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant. Kazune Ezaki, Hiroyuki Koga, Noriko Takeda-Kamiya, Kiminori Toyooka, Takumi Higaki, Shingo Sakamoto, Hirokazu Tsukaya. Frontiers in Plant Science 15 1322223-1322223 2024/4/16

Comprehensive and quantitative analysis of intracellular structure polarization at the apical-basal axis in elongating Arabidopsis zygotes. Yukiko Hiromoto, Naoki Minamino, Suzuka Kikuchi, Yusuke Kimata, Hikari Matsumoto, Sakumi Nakagawa, Minako Ueda, Takumi Higaki. Scientific reports 13(1) 22879-22879 2023/12/18

Gaining new insights in plant biology through human-machine collaboration. Keiji Nakajima, Takumi Higaki, Takashi Ueda, Masahiko Inami. Plant And Cell Physiology 2023/11/10

Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice. Yang Yue, Takashi Hotta, Takumi Higaki, Kristen J Verhey, Ryoma Ohi. Current biology : CB 33(19) 4111-4123 2023/10/9

Smooth Elongation of Pavement Cells Induced by RIC1 Overexpression Leads to Marginal Protrusions of the Cotyledon in Arabidopsis thaliana. Kotomi Kikukawa, Hisako Takigawa-Imamura, Kouichi Soga, Toshihisa Kotake, Takumi Higaki. Plant And Cell Physiology 2023/9/18

The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis. Tomoko Hirano, Kazuo Ebine, Takashi Ueda, Takumi Higaki, Takahiro Watanabe-Nakayama, Hiroki Konno, Hisako Takigawa-Imamura, Masa H Sato. The Plant cell 2023/9/15

Arabidopsis thaliana Subclass I ACTIN DEPOLYMERIZING FACTORs Regulate Nuclear Organization and Gene Expression. Tomoko Matsumoto, Takumi Higaki, Hirotomo Takatsuka, Natsumaro Kutsuna, Yoshiyuki Ogata, Seiichiro Hasezawa, Masaaki Umeda, Noriko Inada. Plant And Cell Physiology 2023/8/18

Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane. Takeshi Nishimura, Shogo Mori, Hiromasa Shikata, Moritaka Nakamura, Yasuko Hashiguchi, Yoshinori Abe, Takuma Hagihara, Hiroshi Y. Yoshikawa, Masatsugu Toyota, Takumi Higaki … Science 2023/8/10

Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells. Haymar Wint, Jianzhen Li, Tadashi Abe, Hiroshi Yamada, Takumi Higaki, Yasutomo Nasu, Masami Watanabe, Kohji Takei, Tetsuya Takeda. Journal of cell science 2023/5/3

At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles. Hirotomo Takatsuka, Takumi Higaki, Masaki Ito. International Journal of Molecular Sciences 24(4) 4143-4143 2023/2/18

Ab-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana. Tomoko Hirano, Ayaka Okamoto, Yoshihisa Oda, Tomoaki Sakamoto, Seiji Takeda, Takakazu Matsuura, Yoko Ikeda, Takumi Higaki, Seisuke Kimura, Masa H. Sato. Scientific Reports 13 2554 2023/1

Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells. Yuji Takihara, Takumi Higaki, Tomomasa Yokomizo, Terumasa Umemoto, Kazunori Ariyoshi, Michihiro Hashimoto, Maiko Sezaki, Hitoshi Takizawa, Toshihiro Inoue, Toshio Suda … Communications Biology 5(1) 776-776 2022/12

Disease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ. Keiko Morotomi-Yano, Yukiko Hiromoto, Takumi Higaki, Ken-Ichi Yano. Scientific reports 12(1) 20627-20627 2022/11/30

Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells. Daichi Yoshida, Kae Akita, Takumi Higaki. Protoplasma 2022/10/11

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Takashi Hotta, Thomas S McAlear, Yang Yue, Takumi Higaki, Sarah E Haynes, Alexey I Nesvizhskii, David Sept, Kristen J Verhey, Susanne Bechstedt, Ryoma Ohi. Current biology : CB 32(18) 3898-3910 2022/9/26

Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins. Takuya Sakamoto, Yuki Sakamoto, Stefan Grob, Daniel Slane, Tomoe Yamashita, Nanami Ito, Yuka Oko, Tomoya Sugiyama, Takumi Higaki, Seiichiro Hasezawa … Nature Plants 8(8) 940-953 2022/8/1

Migration of prospindle before the first asymmetric division in germinating spore of Marchantia polymorpha. Yuuki Sakai, Takumi Higaki, Kimitsune Ishizaki, Ryuichi Nishihama, Takayuki Kohchi, Seiichiro Hasezawa. Plant Biotechnology 39(1) 5-12 2022/3/25

Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis. Emiko Okubo-Kurihara, Ahmed Ali, Mika Hiramoto, Yukio Kurihara, Yasmine Abouleila, Eman Muhammad Abdelazem, Takayuki Kawai, Yuko Makita, Mika Kawashima, Tsuyoshi Esaki, Hiroaki Shimada, Tetsuya Mori, Masami Yokota Hirai, Takumi Higaki, Seiichiro Hasezawa, Yoshihiro Shimizu, Tsutomu Masujima, Minami Matsui. Plant physiology 189(2) 459-464 2022/3/18

Two Kinesin-14A Motors Oligomerize to Drive Poleward Microtubule Convergence for Acentrosomal Spindle Morphogenesis in Arabidopsis thaliana. Takashi Hotta, Yuh-Ru Julie Lee, Takumi Higaki, Takashi Hashimoto, Bo Liu. Frontiers in cell and developmental biology 10 949345-949345 2022

Cell Cycle Synchronization and Time-Lapse Imaging of Cytokinetic Tobacco BY-2 Cells. Keisho Maeda, Takumi Higaki. Methods in molecular biology (Clifton, N.J.) 2382 245-252 2022

Phosphorylation of NONPHOTOTROPIC HYPOCOTYL3 affects photosensory adaptation during the phototropic response. Taro Kimura, Ken Haga, Yuko Nomura, Takumi Higaki, Hirofumi Nakagami, Tatsuya Sakai. Plant physiology 187(2) 981-995 2021/10/5

Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events. Eri Kamon, Chihiro Noda, Takumi Higaki, Taku Demura, Misato Ohtani. Plant biotechnology (Tokyo, Japan) 38(3) 331-337 2021/9/25

Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the Arabidopsis Zygote. Hikari Matsumoto, Yusuke Kimata, Takumi Higaki, Tetsuya Higashiyama, Minako Ueda. Plant & cell physiology 62(8) 1280-1289 2021/6/2

Acentrosomal spindle morphogenesis is dependent on the unconventional TPX2 family protein TPXL3 and a Aurora kinase in Arabidopsis thaliana. Xingguang Deng, Takumi Higaki, Honghui Lin, Yuh-Ru Julie Lee, Bo Liu. 2021/6/1

A Three-Dimensional Scanning System for Digital Archiving and Quantitative Evaluation of Arabidopsis Plant Architectures. Itsuki Kunita, Miyo Terao Morita, Masashi Toda, Takumi Higaki. Plant & cell physiology 62(12) 1975-1982 2021/5/22

Transcriptional regulators involved in responses to volatile organic compounds in plants. Ayumi Nagashima‐KASAHARA, Takumi Higaki, Takao Koeduka, Ken Ishigami, Satoko Hosokawa, Hidenori Watanabe, Kenji Matsui, Seiichiro Hasezawa, Kazushige Touhara. The FASEB Journal 35(S1) 2021/5

The Boundary-Expressed EPIDERMAL PATTERNING FACTOR-LIKE2 Gene Encoding a Signaling Peptide Promotes Cotyledon Growth during Arabidopsis thaliana Embryogenesis. Rina Fujihara, Naoyuki Uchida, Toshiaki Tameshige, Nozomi Kawamoto, Yugo Hotokezaka, Takumi Higaki, Rüdiger Simon, Keiko U Torii, Masao Tasaka, Mitsuhiro Aida. Plant biotechnology (Tokyo, Japan) 38(3) 317-322 2021/4/28

Disruption of actin filaments delays accumulation of cell plate membranes after chromosome separation. Keisho Maeda, Takumi Higaki. Plant signaling & behavior 16(4) 1873586-1873586 2021/4/3

Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules. Takashi Ishida, Haruna Yoshimura, Masatsugu Takekawa, Takumi Higaki, Takashi Ideue, Masaki Hatano, Masayuki Igarashi, Tokio Tani, Shinichiro Sawa, Hayato Ishikawa. Scientific reports 11(1) 6077-6077 2021/3/23

Involvement of the membrane trafficking factor patrol1 in the salinity stress tolerance of arabidopsis thaliana. Fumiya Sato, Koh Iba, Takumi Higaki. Cytologia 86(2) 119-126 2021

シロイヌナズナ草姿の立体再構成—Three-dimensional reconstruction of Arabidopsis plant architecture—特集 植物の柔軟な形態変化を理解するための計測と理論。國田 樹, 金城 愛梨, 戸田 真志, 檜垣 匠。ＰＬＡＮＴ ＭＯＲＰＨＯＬＯＧＹ 33(1) 67-69 2021年

植物感染性線虫の根こぶ形成におけるPUCHI遺伝子の機能解析。鈴木れいら, 檜垣匠, 相田光宏, 久保稔, 澤進一郎。Nematological Research 12(2) 755610-755610 2021年

Metal-Nano-Ink Coating for Monitoring and Quantification of Cotyledon Epidermal Cell Morphogenesis. Kotomi Kikukawa, Kazuki Yoshimura, Akira Watanabe, Takumi Higaki. Frontiers in plant science 12 745980-745980 2021